Publication Date:
2007
Abstract:
ABSTRACT: A case of fatal intoxication from metformin is presented. The decedent was an obese 58-year-old-woman with type II diabetes, in
whom severe lactic acidosis secondary to metformin accumulation was precipitated by acute renal failure. She had been on metformin 500 mg twice
a day. Postmortem blood was deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected into high-performance
liquid chromatography system. Separation was performed on a analytical 125 · 4 mm i.d. RP-8 column. The wavelength was set at 235 nm.
The mobile phase was acetonitrile (40%), sodium lauryl sulfate, and sodium dihydrogen phosphate adjusted to pH 5.1 (60%) at a flow rate of
1.0 mL⁄ min. The concentration of metformin in postmortem blood was 77.3 lg ⁄ mL. The qualitative result was also confirmed by LC⁄APCI
⁄MS⁄MS analysis.
whom severe lactic acidosis secondary to metformin accumulation was precipitated by acute renal failure. She had been on metformin 500 mg twice
a day. Postmortem blood was deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected into high-performance
liquid chromatography system. Separation was performed on a analytical 125 · 4 mm i.d. RP-8 column. The wavelength was set at 235 nm.
The mobile phase was acetonitrile (40%), sodium lauryl sulfate, and sodium dihydrogen phosphate adjusted to pH 5.1 (60%) at a flow rate of
1.0 mL⁄ min. The concentration of metformin in postmortem blood was 77.3 lg ⁄ mL. The qualitative result was also confirmed by LC⁄APCI
⁄MS⁄MS analysis.
CRIS type:
1.1 Articolo in rivista
List of contributors:
Saligari, Elisa Agnese; Gambero, V; Dellacqua, L; Farè, F; Fidani, M; Froldi, R.
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