Androgen receptor is expressed in both X- and Y-carrier human spermatozoa

OBJECTIVE:

To quantify the amount of AR messenger RNA (mRNA) and to analyze the presence and functionality of the AR protein in X- and Y-carrier human spermatozoa.
DESIGN:

A molecular and cellular research study.
SETTING:

Academic research clinic and laboratories.
PATIENT(S):

Ten normozoospermic volunteers.
INTERVENTION(S):

Sperm were analyzed for expression of AR mRNA and protein. The functionality of AR protein was assessed after incubation with 1 nM of synthetic androgen.
MAIN OUTCOME MEASURE(S):

Reverse-transcription polymerase chain reaction (PCR), real-time PCR, Western immunoblotting, confocal immunofluorescence, and fluorescence in situ hybridization analysis were performed.
RESULT(S):

A significant amount of AR mRNA (around 30% of that found in the testis) was found in sperm. Androgen receptor protein was found in both X- and Y-carrier spermatozoa and translocates into the nucleus in the presence of synthetic androgen.
CONCLUSION(S):

A functional AR is expressed in human sperm. In view of the fact that AR protein is found in both X- and Y-carrier spermatozoa, it most probably is translated in earlier steps of spermatogenesis and moves to Y-carrier spermatids through the cytoplasmic bridges.