Publication Date:
2010
Abstract:
Because of the increased use of circular dichroism (CD) spectroscopy
as a routine technique by nonspecialists to determine the conformational/configurational
properties of biomolecules, we have decided to present here some criteria to accurately
check the ordinate scale calibration of a CD spectrometer particularly in the critical lowwavelength
UV region, to understand, and correct, where possible, the potential limitations
coming from the hardware. We also analyze some wavelength calibration methods,
and some standards for the CD-scale calibration, and we discuss the critical characteristics
of current instrumentation affecting measurements. The example of the bovine catalase
CD spectrum is considered.
as a routine technique by nonspecialists to determine the conformational/configurational
properties of biomolecules, we have decided to present here some criteria to accurately
check the ordinate scale calibration of a CD spectrometer particularly in the critical lowwavelength
UV region, to understand, and correct, where possible, the potential limitations
coming from the hardware. We also analyze some wavelength calibration methods,
and some standards for the CD-scale calibration, and we discuss the critical characteristics
of current instrumentation affecting measurements. The example of the bovine catalase
CD spectrum is considered.
CRIS type:
1.1 Articolo in rivista
List of contributors:
Castiglioni, Ettore; Albertini, P; Abbate, Sergio
Published in: