An operative approach to correct CD spectra distortions due to absorption flattening
Academic Article
Publication Date:
2008
Abstract:
We discuss the influence of absorption flattening (AF) on CD spectra,
aiming at finding a simple way to compensate the induced distortions. A simple algebraic
formalism is proposed based on an unconventional measuring approach, which makes
use of a commercial unit. Validation of the proposed compensation method for AF is conducted
not only on the already studied CD Vis spectral region of a Co chiral complex
with the use of an AF emulator, but also on the far UV CD spectra of poly-L-glutamic acid
suspensions with different turbidity levels. Comparison with some correction factors proposed
in the literature is presented.
aiming at finding a simple way to compensate the induced distortions. A simple algebraic
formalism is proposed based on an unconventional measuring approach, which makes
use of a commercial unit. Validation of the proposed compensation method for AF is conducted
not only on the already studied CD Vis spectral region of a Co chiral complex
with the use of an AF emulator, but also on the far UV CD spectra of poly-L-glutamic acid
suspensions with different turbidity levels. Comparison with some correction factors proposed
in the literature is presented.
CRIS type:
1.1 Articolo in rivista
Keywords:
circular dichroism; absorption flattening; poly-L-(glutamic acid); PGA
List of contributors:
Castiglioni, E; Lebon, France; Longhi, Giovanna; Gangemi, Roberto; Abbate, Sergio
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