Performance evaluation of the automated NucliSens EasyMAG nucleic acid extraction platform in comparison to QIAamp Mini Kit from clinical specimens.
Articolo
Data di Pubblicazione:
2009
Abstract:
The performance of the NucliSens easyMAG platform for the extraction of nucleic acid from different clinical specimens was compared
with a manual procedure. A total of 308 specimens were analyzed: 209 plasma samples collected for virus detection and quantification of
cytomegalovirus (CMV) and Epstein–Barr virus (EBV) (n = 70), and 29 for HIV genotyping for drug resistance. Linearity of extraction was
tested on dilution series of CMVand EBV; the correlation coefficient (R2) for standard curves based on repeated extraction runs was 0.99 for
CMV and EBV. Inter- and intrarun variability was in accordance with previous studies, and the correlation between automated and manual
extraction was very high. The concordant results were 95.7% for CMV and 100% for EBV. The results of sequence analysis for HIV drug
resistance showed a concordance in 24 of 29 specimens. The NucliSens easyMAG is extremely easy to perform, is automated, and resulted in
a strong reduction of hands-on time compared with manual protocol.
with a manual procedure. A total of 308 specimens were analyzed: 209 plasma samples collected for virus detection and quantification of
cytomegalovirus (CMV) and Epstein–Barr virus (EBV) (n = 70), and 29 for HIV genotyping for drug resistance. Linearity of extraction was
tested on dilution series of CMVand EBV; the correlation coefficient (R2) for standard curves based on repeated extraction runs was 0.99 for
CMV and EBV. Inter- and intrarun variability was in accordance with previous studies, and the correlation between automated and manual
extraction was very high. The concordant results were 95.7% for CMV and 100% for EBV. The results of sequence analysis for HIV drug
resistance showed a concordance in 24 of 29 specimens. The NucliSens easyMAG is extremely easy to perform, is automated, and resulted in
a strong reduction of hands-on time compared with manual protocol.
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Perandin, Francesca; POLLARA C., Patrizia; Gargiulo, Franco; Bonfanti, Carlo; Manca, Nino
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